Advances in ISSN: 2373-6402APAR

Plants & Agriculture Research
Research Article
Volume 2 Issue 2 - 2015
Variability in Growth and Yield Response of Maize Genotypes at Elevated CO2 Concentration
Vanaja M1*, Maheswari M2, Jyothi Lakshmi N1, Sathish P3, Yadav SK4, Salini K5, Vagheera P6, Vijay Kumar G7 and Abdul Razak7
1Plant physiology, Central Research Institute for Dryland Agriculture, India
2Head, Division of Crop Sciences, India
3Technical Assistant, India
4Biochemistry, India
5Plant Breeding, India
6Research Associate, India
7Senior Research Fellow, India
Received:January 21, 2015 | Published: March 18, 2015
*Corresponding author: Vanaja M, Principal Scientist (Plant Physiology), Division of Crop Sciences, Central Research Institute for Dryland Agriculture, Santoshnagar, Hyderabad 500 059, India, Tel: 91 40 24530161; 09247349409; Fax: 91 40 24531802; Email: @, @
Citation: Vanaja M, Maheswari M, Jyothi Lakshmi N, Sathish P, Yadav SK, et al. (2015) Variability in Growth and Yield Response of Maize Genotypes at Elevated CO2 Concentration. Adv Plants Agric Res 2(2): 00042. DOI: 10.15406/apar.2015.02.00042


Three contrasting maize (Zea mays L.) genotypes- DHM-117 (single cross hybrid), Varun (synthetic) and Harsha (composite) with different yield potentials were selected to assess their growth and yield performance at ambient (390ppm) and elevated (550ppm) CO2 condition in Open Top Chamber (OTC) facility. The phenology, biomass accumulation, grain yield and HI was quantified of these three maize genotypes at both CO2 levels. The phenology of flowering was early by 1.5 to 2 days, while the anthesis silking interval (ASI) was not influenced by elevated CO2 in DHM-117 and Varun, where as it was reduced by two days in Harsha. Response of selected three maize genotypes was different to elevated CO2 (550ppm) condition in terms of biomass, grain yield and HI. The improvement in biomass ranged from 32% to 47%, grain yield 46% to 127% with 550ppm CO2 as compared with ambient control. The improvement in grain yield was due to increased grain number (25-72%) as well as improved test weight (8-60%). The overall response of less efficient maize genotype Harsha with elevated CO2 concentration was found to be significantly high especially the grain yield and its components. Elevated CO2 also improved the maize HI (11% to 68%) indicating that influence of elevated CO2 was there on partitioning of biomass of this C4 crop.
Keywords: Maize; Elevated CO2; Genotypes; ASI; Grain yield; Grain number; HI


The changing climatic conditions are expected to increase the atmospheric CO2 concentration, temperatures and alter the precipitation pattern. Atmospheric CO2 concentration is predicted to reach 550ppm by 2050, and probably exceed 700ppm by the end of this century [1]. These changes are anticipated to affect the production and productivity of agricultural crops and influence the future food security. The impact analysis of climate change on global food production discloses a 0.5% decline by 2020 and 2.3% by 2050 [2,3]. The development of climate ready germplasm to offset these losses is of the upmost importance [4].

The C4 grass maize (Zea mays L.) is the third most important food crop globally in terms of production and its demand is predicted to increase by 45% from 1997 to 2020 [5]. Studies with maize response to double the ambient CO2 showed varying effects on growth ranging from no stimulation of biomass [6] to 50 % stimulation [7]. These studies reveal that C4 plants do have the potential to respond to elevated CO2. The basis for the observed enhancement of growth of C4 plants under elevated CO2 is not as clear as in C3; plants. The present study was aimed to assess the response variability of maize genotypes at elevated CO2 condition in terms of phenology, biomass and yield components.

Materials and Methods

The seed material of the maize genotypes DHM-117, Varun and Harsha were obtained from DMR Regional station at Hyderabad and raised in open top chambers (OTCs) at ambient (390ppm) and elevated (550ppm) CO2 levels during post rainy season (Rabi) 2012. The OTCs having 3m x 3m x 3m dimensions lined with transparent PVC (polyvinyl chloride) sheet having 90% transmittance of light were used. The elevated CO2 of 550ppm was maintained in two OTCs and other two OTCs without any additional CO2 supply served as ambient control. The CO2 concentrations within the OTCs were maintained and monitored continuously throughout the experimental period as illustrated by Vanaja et al. [8].

Each chamber had 6 plants of each genotype planted in two rows of 1.0m with 0.35m spacing within row and 0.75m between rows. The recommended dose of fertilizers 60 kg N ha-1 and 60 kg P ha-1 as diammonium phosphate, 30 kg K ha-1 as muriate of potash was applied as basal dose; second dose of 30 kg N ha-1 at knee- high stage and third dose of 30 kg N ha-1 as urea and 30 kg potassium ha-1 as muriate of potash was side dressed at tasseling stage. The crop was irrigated at regular intervals and maintained pest and disease free with plant protection measures.

The phenological observations such as days to 50% tasseling, anthesis and silking and maturity were recorded. At harvest the observations on plant height, total biomass, stover weight, cob weight, grain yield, test weight and other yield contributing traits were recorded. The analysis of variance (ANOVA) was carried out to assess the significance of CO2 levels and genotypes and their interaction.

Results and Discussion

The analysis of variance (ANOVA) revealed that the selected three maize genotypes- DHM-117, Varun and Harsha recorded significant difference (p < 0.01) for plant height, total biomass, stover weight, cob weight, grain yield, test weight and HI. The CO2 levels were significant for total biomass, cob weight, grain yield, grain number and test weight at p < 0.01 level and for plant height and harvest index at p < 0.05 level (Table 1), whereas the interaction of genotypes x CO2 levels was significant only for test weight.

The plant height of all the maize genotypes showed a significant (p < 0.01) increase with enhanced CO2 concentration (550ppm) as compared with ambient grown plants (Table 1). Driscoll et al. [9] observed increase in maize plant height by 23% at 700ppm CO2 and affirmed that being a C4 crop, maize plant can show improved performance to increased CO2 concentration. Elevated CO2 also influenced the phenology of flowering in maize and it was observed that day to 50% tasseling, anthesis and silking was early by 1.5 to 2 days as compared with ambient controls (Figure 1). However, anthesis-silking interval (ASI) in DHM-117 and Varun was not influenced by elevated CO2 as both anthesis and silking were early, whereas in Harsha, elevated CO2 could reduce only the days to silking and not anthesis there by ASI was shortened to the extent of two days. In the life cycle of plant, the flowering time is very critical stage and in many crops it determines the number of seeds and final yield [10] and the environmental conditions which affect the plant growth tend to influence the flowering dynamics [11]. Review of 60 studies on flowering time and elevated atmospheric CO2 by Springer and Ward [12] revealed that this response is crop and variety specific. The enhanced CO2 condition reduced the days to initiation and 50% flowering in castor bean [13], whereas a delay in phenology of flowering was observed in soybean [14]. Leakey et al. [15] from their FACE experiments reported that 550ppm CO2 didn’t influenced the duration of anthesis and silking of maize cv 34B43 (Pioneer Hi-Bred International).

Figure 1: Days to tasseling (T), anthesis (A) and silking (S) of three maize genotypes- DHM-117, Varun and Harsha at ambient (390ppm) and elevated (550ppm) CO2 conditions.

The response of selected maize genotypes was different to elevated CO2 (550ppm) condition in terms of total biomass, grain yield and HI. Enhanced CO2 concentration significantly improved the total biomass as compared with ambient condition and the response was maximum with Varun (47%) followed by Harsha (34%) and DHM-117 (32%). Studies on impact of elevated CO2 on maize crop revealed varying effects from no stimulation of biomass [6] to 3-6% [16], 20% [17,18], 24% [19], 36% [20] and up to 50% [7]. This differences in magnitude of response of maize to elevated CO2 could be due to genotypic variability [21], strength of source and sink, management of the crop such as water and nutritional status, duration of exposure, light intensity, temperature, and even pot size [22,23].

The increase in biomass can be explained by the ability of the high CO2 grown plants to maintain elevated photosynthetic rates and there was a 1.5 to 2 fold increase in internal CO2. Ghannoum et al. [24] proposed two major mechanisms that may be responsible for increasing C4 plant growth under elevated CO2. The first potential mechanism operates through CO2-induced increases in net photosynthetic rates and second mechanism deals with CO2-induced reductions in stomatal conductance which can improve overall plant water relations and facilitate greater biomass production. In addition, reductions in transpirational water loss may slightly increase leaf temperature, thereby stimulating rates of photosynthesis and biomass production. Increased photosynthetic rate to synthesize the more sucrose and starch, and to utilize these end products of photosynthesis to produce extra energy by respiration, may contribute to the enhanced growth of maize under elevated CO2.

The impact of elevated CO2 was observed to be different in enhancing the vegetative and reproductive biomass of selected maize genotypes. With enhanced CO2 greater vegetative growth was recorded by Varun (36%), whereas reproductive biomass by Harsha (94%). It is interesting to observe that in all the genotypes the increased response of reproductive biomass was much higher with enhanced CO2 condition than vegetative biomass and indicating its function in triggering the partitioning of biomass more towards cob or grain weight (Figure 2). The improved grain yield due to 550ppm CO2 was 46% in DHM-117, 61% in Varun and 127% in Harsha as compared with respective ambient control (Table 1). The improvement in grain yield was contributed by both increased grain number to the extent of 34%, 25% and 72% as well as enhanced test weight by 8%, 29% and 60% in DHM-117, Varun and Harsha respectively. Elevated CO2 also significantly improved the HI of maize genotypes to the extent of 11% (DHM-117 and Varun) to 68% (Harsha). The simulation study using CropSyst model on the impact of elevated CO2 on major cereal crops revealed that maize response was more than C3 rice and wheat as well as C4 pearl millet [25] and even the increase in yield was observed up to 3°C rise in temperature under doubled CO2 situation. It was concluded that the improved response of maize being a C4 crop could be due to more efficient use of increased CO2 than the other C3 crops.

Figure 2: The impact of elevated CO2 (550ppm) on increase (%) of biomass, grain yield and HI of three maize genotypes- DHM-117, Varun and Harsha over ambient control (390ppm).
Significant differences
G x CO2
Plant height (cm)
292 (3.9)
283 (11.4)
237 (4.2)
Total biomass (g)
Cob dry weight (g)
92.6 (106)
Stover biomass (g)
233.9 (26)
130.9 (39)
102.1 (2.7)
Grain yield (g)
115.3 (46)
77.3 (126.8)
Number of grains/cob
522 (34)
431 (24.5)
484 (71.5)
100 grain weight (g)
17.4 (60)
Harvest Index (%)

Table 1: Growth, biomass and yield traits under ambient (390ppm) and elevated (550ppm)CO<sub>2</sub> conditions of maize genotypes DHM-117, Varun and Harsha.

*, ** Significant at P< 0.05 and 0.01, respectively; ns indicates non-significant; G- genotypes, CO2- CO2 levels aCO2- ambient (390ppm) and eCO2- elevated (550ppm) CO2 conditions; Values in parenthesis indicate the % increase over ambient


The evaluation of maize genotypes at elevated CO2 (550ppm) for their biomass and yield revealed that maize crop though having C4 photosynthetic pathway was able to respond positively with enhanced atmospheric CO2 concentration. It is also evident that there is a significant variability between maize genotypes in response to elevated CO2. The positive and significant response of elevated CO2 on maize HI was due to higher partitioning of biomass towards reproductive parts than vegetative parts makes this crop more climate resilient.


The present work at CRIDA, Hyderabad was funded by Indian Council of Agriculture Research (ICAR) under National Initiative on Climate Resilient Agriculture (NICRA).


  1. IPCC (2007) Summary for policy makers. In: Solomon SD, et al. (Eds.), Climate change 2007: The physical science basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, United Kingdom.
  2. Ainsworth EA, Long SP (2005) What have we learned from 15 years of free-air CO2 enrichment (FACE)? A meta-analytic review of the responses of photosynthesis, canopy properties and plant production to rising CO2. New Phytol 165(2): 351-371.
  3. Calzadilla A, Zhu T, Rehdanz K, Tol RSJ, Ringler C (2013) Economy wide impacts of climate change on agriculture in Sub-Saharan Africa. Ecological Economics 93: 150-165.
  4. Cairns JE, Sonder K, Zaidi PH, Verhulst N, Mahuku G, et al. (2012) Maize Production in a Changing Climate: Impacts, Adaptation, and Mitigation Strategies. Advances in Agronomy 114: 1-65.
  5. Young KJ, Long SP (2000) Crop ecosystem responses to climatic change: maize and sorghum. In: Reddy KR & Hodges HF (Eds.), Climate change and global crop productivity, CABI International, Oxon, United Kingdom, pp. 107-131.
  6. Hunt R, Hand D, Hannah M, Neal A (1991) Response to CO2 enrichment in 27 herbaceous species. Functional Ecology 5: 410-421.
  7. Rogers HH, Dahlman RC (1993) Crop responses to CO2 enrichment. CO2 and biosphere. Advances in vegetation science 14: 117-131.
  8. Vanaja M, Maheswari M, Ratnakumar P, Ramakrishna YS (2006) Monitoring and controlling of CO2 concentrations in open top chambers for better understanding of plants response to elevated CO2 levels. Indian J Radio & Space Phys 35: 193-197.
  9. Driscoll SP, Prins A, Olmos E, Kunert KJ, Foyer CH (2006) Specification of adaxial and abaxial stomata, epidermal structure and photosynthesis to CO2 enrichment in maize leaves. J Exp Bot 57(2): 381-390.
  10. Craufurd PQ, Wheeler TR (2009) Climate change and the flowering time of annual crops. J Exp Bot 60(9): 2529-2539.
  11. Borras G, Romagosa I, van Eeuwijk F, Slafe GA (2009) Genetic variability in the duration of pre-heading phases and relationships with leaf appearance and tillering dynamics in a barley population. Field Crop Research 113(2): 95-104.
  12. Springer C, Ward J (2007) Flowering time and elevated atmospheric CO2. New Phytol 176(2): 243-255.
  13. Vanaja M, Raghu Ram Reddy P, Maheswari M, Jyothi Lakshmi N, Yadav SK, et. al. (2009) Impact of elevated carbon dioxide on growth and yield of castor bean. In: Aggarwal PK (Ed.) Global Climate Change and Indian Agriculture- Case Studies from the ICAR Network Project. Indian Council of Agricultural Research, India, pp. 32-34.
  14. Castro JC, Dohleman FG, Bernacchi CJ, Long SP (2009) Elevated CO2 significantly delays reproductive development of soybean under Free-Air Concentration Enrichment (FACE). J Exp Bot 60(10): 2945-2951.
  15. Leakey A, Uribelarrea M, Ainsworth E, Naidu S, Rogers A, et al. (2006) Photosynthesis, productivity and yield of maize are not affected by open-air elevation of CO2 concentration in the absence of drought. Plant Physiol 140(2): 779-790.
  16. Ziska LH, Bunce JA (1997) Influence of increasing CO2 concentration on the photosynthetic and growth stimulation of selected C4 crops and weeds. Photosynthesis Research 54: 199-207.
  17. Maroco JP, Edwards GE, Ku MS (1999) Photosynthetic acclimation of maize to growth under elevated levels of carbon dioxide. Planta 210(1): 115-125.
  18. Wong SC (1979) Elevated atmospheric partial pressure of CO2 and plant growth. I. Interaction of nitrogen nutrition and photosynthetic capacity in C3 and C4 plants. Oecologia 44(1): 68-74.
  19. Carlson R, Bazzaz F (1980) The effects of elevated CO2 concentrations on growth, photosynthesis, transpiration and water use efficiency of plants. In: Singh J & Deepak A (Eds.), Environmental and climatic impact of coal utilization. Academic Press, New York, USA, pp. 655.
  20. Morison J, Gifford R (1984) Plants growth and water use with limited water supply in high CO2 concentrations. II. Plant dry weight, partitioning and water use efficiency. Australian J Plant Physiology 11(5): 375-384.
  21. Berg A, de Noblet-Ducoudre N, Sultan B, Lengaigne M, Guimberteau M (2013) Projections of climate change impacts on potential C4 crop productivity over tropical regions. Agricultural and Forest Meteorology 170: 89-102.
  22. Sage RF (1994) Acclimation of photosynthesis to increasing atmospheric CO2: the gas exchange perspective. Photosynth Res 39(3): 351-368.
  23. Drake BG, Gonzalez-Meler MA, Long SP (1997) More efficient plants: a consequence of rising atmospheric CO2? Annu Rev Plant Phys 48: 609-639.
  24. Ghannoum O, Von Caemmerer S, Ziska LH, Conroy JP (2000) The growth response of C4 plants to rising atmospheric CO2 partial pressure: a reassessment. Plant Cell and Environment 23(9): 931-934.
  25. Tripathy R, Ray SS, Singh AK (2009) Analyzing the impact of rising temperature and CO2 on growth and yield of major cereal crops using simulation model. In: Panigrahy S, et al. (Eds.), ISPRS Archives XXXVIII-8/W3 Workshop Proceedings: Impact of Climate Change on Agriculture, India.
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