Journal of ISSN: 2377-4312JDVAR

Dairy, Veterinary & Animal Research
Research Article
Volume 2 Issue 6 - 2015
Studies on Some Biochemical Constituents of Mithun Semen
Bhattacharyya HK1, Goswami BK1, Bujarbaruah KM1, Deka BC2 and Biswas RK2
1Assam Agricultural University, India
2Department of Gynecology and Obstetrics, Assam Agricultural University, India
Received: October 15, 2015| Published: November 6, 2015
*Corresponding author: Hiranya Kumar Bhattacharyya, Senior Scientist, Krishi Vigyan Kendra (Farm Science Centre), Assam Agricultural University (AAU), P.O. Lahoal, Post Box No: 24, Dibrugarh-786010, Assam, India, Tel: +91-9435564391; Email:
Citation: Bhattacharyya HK, Goswami BK, Bujarbaruah KM, Deka BC, Biswas RK (2015) Studies on Some Biochemical Constituents of Mithun Semen. J Dairy Vet Anim Res 2(6): 00055. DOI: 10.15406/jdvar.2015.02.00055


A total of 15 semen samples were collected from 3 Mithun bulls reared at the farm of National Research Centre on Mithun (NRCM), Nagaland to measure cholesterol (mg/ dl), total protein (g/ dl), glutamic oxaloacetic transaminase (IU/ L), glutamic pyruvic transaminase (IU/ L), lactic dehydrogenase (IU/ L), glucose (mg/ dl), sodium (mmol/L) and potassium (mmol/L) and their mean concentrations were recorded as 23.94±6.37, 3.57±0.64, 31.61±7.30, 15.58±1.50, 78.36±6.46, 32.57±9.78, 300.30±40.30 and 16.05±1.30, respectively.

Keywords: Seminal constituents; Mithun; Male reproduction


The Mithun (Bos frontalis), a semi-wild animal, which is yet to be fully domesticated, is mainly confined in India, Bhutan, Myanmar, Bangladesh and hilly tracts of China and Malaysia. In India it is only found in the hilly tracts of four states of North Eastern India namely Arunachal Pradesh, Nagaland, Manipur and Mizoram, in descending order of population. Mithun plays a significantly important role in the socio-economic and religious life of the people of this region living at an altitude of 2700-3000 meter above sea level [1]. It is very much important to know the biochemical constituents of seminal plasma of normal bulls as any alterations in its normal concentration may reflect some abnormalities in genital system. Moreover it will help in conducting future research activities related to semen processing and conservation. Till date no report is available on biochemical constituents of Mithun semen. In our earlier papers we described detailed collection procedure and characterization of semen collected by both massage and artificial vagina method in Mithun bulls [2,3]. Now, we wish to report on some seminal biochemical constituents, in this valuable animal species.

Materials and Methods

Semen samples (15 no’s) were collected from 3 Mithun bulls (5 samples from each bull), aged 2.5 to 4 years, maintained at the farm of National Research Centre on Mithun (NRCM), Nagaland, along with other Mithun of the herd purchased few months earlier from different hilly tracts of North-Eastern states of India. The bulls were maintained under semi-intensive system of management providing concentrate feed in the morning and evening and allow them to graze in the intervening period. The bulls were giving training for semen collection for few months before they were actually used for semen collection using both estrous and anestrous female as teaser. Semen was collected with the help of artificial vagina having an internal temperature of 42oC and 46oC (average 44oC), using estrous female as teaser, whenever available in the herd [3], as bulls did not mount anestrous female. Within an hour of collection of semen, seminal plasma was removed by centrifuging semen at 3000 rpm for 30 minutes. Cholesterol (Ch), total protein (TP), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), glucose, sodium (Na) and potassium (K) were estimated by standard calorimetric method in blood chemistry analyzer (RA-50 machine manufactured by Bayer Diagnostic, Bombay, India) using commercially available kit by the instructions provided in the leaflet supplied inside the kit. Finally F test was used to determine bull variations, if any, amongst the different biochemical constituents.

Results and Discussion

The mean values of Cholesterol (mg/dl), TP (g/dl), GOT (U/L), GPT (U/L), LDH (U/L), glucose (mg/dl), Na (mmol/L) and K (mmol/L) were recorded as 23.94±6.37, 3.57±0.64, 31.61±7.30, 15.58±1.50, 78.36±6.46, 32.57±9.78, 300.30±40.30 and 16.05±1.30, respectively (Table 1).


Animal 1

Animal 2

Animal 3


F - value






Cholesterol (mg/dl)







Total Protein (g/dl)




























Glucose (mg/dl)







Na (mmol/L)







K (mmol/L)







Table 1: Animal variations with respect to constituents of seminal plasma in Mithun.

The mean values of cholesterol recorded in Mithun semen were lower than the values recorded for cattle, buffalo [4,5] and goat semen [6]. This might be due to the species variation.

The present finding with respect to TP simulates the finding of Ezekwe & Orzi [7] in domestic bulls. However, the value recorded in the present study was much higher than the values recorded by Buruiana et al. [8] and lesser than the values recorded by Nafornita et al. [9] in domestic cow bulls.

The mean level of GOT recorded in Mithun semen was higher than that reported in domestic bulls [10,11]. Some earlier reports indicated much higher level of GOT content in domestic bull semen [8,9,12]. The GPT value recorded in the study was in agreement with one report in domestic bulls [9]. However, lower [11] and higher [10,12] values were also reported in domestic bulls. In the present study, GOT level was found to be higher than GPT level and the ratio was almost 2:1. Earlier workers also recorded higher GOT level than GPT level in bull semen [12,13]. The LDH level recorded in Mithun semen was lower than the values recorded in Holstein Friesian [11] and Punganur bull [14]. In bulls, all these three enzymes are correlated with several factors including species, sperm concentration, sperm motility, live and dead sperm count, percent abnormal spermatozoa etc and their increased concentration in seminal plasma reflects spermatozoal damage [14]. In Mithun, average sperm concentration (million/mL), initial sperm motility (%), live sperm count (%) and percent abnormal spermatozoa (%) were recorded as 710.8±66.90, 78.6±2.60, 80.7±2.20 and 80.6±0.2, respectively [3].

In the present study sodium content was found much higher than the potassium content. This simulates the earlier finding in cow bull’s seminal plasma that sodium is in excess of potassium [15].

The GPT, LDH, Glucose and K content was significantly varied between the three Mithun bulls; but, bull variation was not observed among the other constituents of seminal plasma. This may be due to species and or individual variation only. However, future research is required to know much on these aspects and also to have some other possible explanation for getting such discrepancies of biochemical constituents found in individual Mithun bulls.

Different biochemical constituents of Mithun semen were generally within the range reported for fertile domestic cow bulls and hence it is concluded that Mithun semen has the potential to result in fertile mating through artificial insemination too.


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