Journal of ISSN: 2378-3184JAMB

Aquaculture & Marine Biology
Research Article
Volume 4 Issue 3 - 2016
Effect of Betaine Hydrochloride as Feed Attractant on Growth, Survival and Feed Utilization of Common Carp, Cyprinus carpio
Shivananda Murthy H*, AkshayaManai and PrakashPatil
Department of Aquaculture, Animal and Fisheries Sciences University, India
Received: June 05, 2016| Published: July 08, 2016
*Corresponding author: Shivananda Murthy H, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575002, India, Email:
Citation: Murthy HS, Manai A, Patil P (2016) Effect of Betaine Hydrochloride as Feed Attractant on Growth, Survival and Feed Utilization of Common Carp, Cyprinus carpio. J Aquac Mar Biol 4(3): 00083. DOI: 10.15406/jamb.2016.04.00083

Abstract

The efficiency of betaine as a feed attractant on growth, survival and feed utilization of common carp (Cyprinus carpio) was evaluated in this study. Betaine was incorporated at 0.25% and 0.50% in a 30% protein diet, basal diet without supplementation of betaine served as control. The experimental diets were fed to common carp fingerlings for 60 days in fiber-glass tanks measuring 1.25 x 0.5 x 0.5m. Feed was given twice daily at a rate of 5% of the body weight during the first 15 days and 3% afterwards.Diet supplemented with 0.25% betaine fed common carps showed higher growth in terms of specific growth rate, survival, and food conversion rate and protein efficiency ratio.

Keywords: Betaine; Feed attractant; Growth; Feed utilization; Cyprinus carpio

Abbreviations

NFE: Nitrogen Free Extractives; DMPT: Dimethyl- Beta-Propiothetine

Introduction

Aquaculture is one of the fastest growing sectors in the world. It is mainly contributing to the increase in export, earning, income, employment, food and nutritionally security for the developing countries. Freshwater aquaculture in India has evolved from the stage of a domestic activity in eastern Indian states like West Bengal and Orissa to that of an organized Industry in recent years with the states like Andhra Pradesh, Punjab, Haryana, Uttarakhand and other states, taking up fish culture on a commercial scale [1]. However, the adequate nutrition is a pre-requisite for optimum growth and survival of farmed fishes. The success of farming operations depends on the availability and supply of nutritionally balanced and acceptable diet. It is well known that proper nutrition is one of the most important factors which influence the ability of an animal to attain genetic potential for growth, reproduction and longevity [2]. Various studies have been conducted to maximize the feed intake by using feed additives, feed attractants and stimulants which have appeared as a boon in aquaculture which not only enhances the feed intake and growth but also help in weaving of hatchlings of marine and freshwater fish for artificial diet [3,4]. Feeding in fish, as in other groups of animals is an important function of life. Many sensory systems contribute to fish feeding behavior. Chemo attraction and chemo stimulation facilitate the initial location (olfactory) and final consumption (gustatory response) of food in the feeding process of fish. Growth performance may be improved by using feeding stimulants in order to increase food intake [5]. Feed attractants or chemo stimulants are therefore generally included in feeds or especially slow feeding species. The use of feed attractants in manufactured aqua feeds has received considerable attention in the recent years. The reason behind their use has been to improve dietary food intake and at the same time by promoting quicker food intake [6]. The time interval between feed offered and intake by the animal is minimized in water and thereby prevent the leaching of water soluble nutrients. Further, attractants provide additional nutrients for protein and energy metabolism so that aqua feeds are ingested with minimum wastage and maximum feed efficiency, which also helps to reduce water pollution [7]. Chemo reception is an important component of food detection in many aquatic animals including fish and shellfish. This has been demonstrated repeatedly by conducting behavioral studies using the amino acid and related compounds as potent feeding stimulants. Fish locate food stuff by following chemical signals that are produced by the respective prey or food, and the olfaction in fish, takes place entirely in the aquatic environment [8]. The carrier of stimulant chemical molecules is water, thus chemicals that are detected by olfaction of fish need to be volatile, but must also be soluble in water [4].

Common carp, Cyprinus carpio, one of the most important fresh water cultivable speciesis benthic, omnivorous, does well in muddy and eutrophic water with soft sediments. It enjoys the nationwide distribution in India and occupies 3rd place in the world aquaculture production after silver carp and grass carp [2]. It can be cultured in monoculture particularly by small and artisan farmers in rural aqua farming andalso in polyculture with Indian major carps, as it readily accepts pellet feeds and grows fast. It has major advantages over the other carp species that too in a tropical country like India because it can be bred almost throughout the year and has a shorter generation time, andalso grow much faster than the major carps such as rohu and mrigal, but its growth is almost equal to that of catla [9]. Improvement in feed utilization helps to reduce feed wastage and feed costs. In this direction, feed attractants play role in effective feed utilization by fish, in particular common carp.. The present study was taken up to know the effect of betaine hydrochloride as feed attractant on growth, survival and feed utilization in common carp, Cyprinuscarpio.

Materials and Methods

Experimental animals

Common carp (Cyprinuscarpio) fry weighing 1 g (+- 0.2) procured from a Government Fish Seed Farm at the Bhadra Reservoir Project in Shivamogga, to the fish farm of the College of Fisheries in Mangaluru, were acclimatized to the pellet diet containing 30% protein for a period of 15 days.

Formulation, preparation and proximate composition of experimental diets

Experimental diets were prepared incorporating betaine hydrochloride at two graded levels namely 0.25 (T1) and 0.5% (T2). Diet without betaine hydrochloride served as control (T0). The basal diet with a crude protein content of 30%, using fish meal and groundnut cake as major protein sources was formulated according to square method [10]. All the diets were analyzed for proximate composition according to [11]. Moisture content was estimated by heating samples at 105 °C for 30 minutes and then cooled and weighed till a constant weight was obtained. Crude protein was analyzed using Kjeltec System, fat content by Soxtech System and fiber content using Fibretech System. Nitrogen free extractives (NFE) were calculated by the difference method [12].

Experimental system

The experiment was carried out in fiber-glass aquarium tanks measuring 1.25 x 0.5 x 0.5m at the indoor field laboratory of the College of Fisheries, Mangaluru. The aquaria were cleaned and filled with water from a nearby perennial open well. Uniform sized common carp fry (0.36 g) were stocked at 15 numbers per aquarium in three replicate groups and were fed twice daily at the rate of 5% of the body weight during the first 15 days and 3% thereafter. Unconsumed feed was siphoned out daily in the morning before offering the feed. After every sampling, the amount of feed given was adjusted based on the weight increase of the fish.

Water quality analysis

Water from all the experimental aquaria was analyzed every week. A digital portable kit model CK 704 was used to measure pH and temperature. Dissolved oxygen was estimated by Winkler’s method. Total alkalinity, ammonia and free carbon dioxide were determined by standard methods [13]. The evaporation loss and deterioration in water quality due to accumulation of metabolites was overcome by periodic partial exchange of water regularly.

Growth studies

Fishes in each tank were sampled fortnightly and individually measured their weight and length. The growth performance of the experimental animals was assessed at the end by calculating weight gain and percentage of survival using the following formula:

SGR= Log final weight ( g )Log initial weight ( g ) Number of days ×100 MathType@MTEF@5@5@+= feaagKart1ev2aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq=Jc9 vqaqpepm0xbba9pwe9Q8fs0=yqaqpepae9pg0FirpepeKkFr0xfr=x fr=xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaqcfaOaam4uai aadEeacaWGsbGaeyypa0ZaaSaaaeaaqaaaaaaaaaWdbiaadYeacaWG VbGaam4zaiaacckacaWGMbGaamyAaiaad6gacaWGHbGaamiBaiaacc kacaWG3bGaamyzaiaadMgacaWGNbGaamiAaiaadshacaGGGcWaaeWa a8aabaWdbiaadEgaaiaawIcacaGLPaaacqGHsislcaWGmbGaam4Bai aadEgacaGGGcGaamyAaiaad6gacaWGPbGaamiDaiaadMgacaWGHbGa amiBaiaacckacaWG3bGaamyzaiaadMgacaWGNbGaamiAaiaadshaca GGGcWaaeWaa8aabaWdbiaadEgaaiaawIcacaGLPaaaa8aabaWdbiaa d6eacaWG1bGaamyBaiaadkgacaWGLbGaamOCaiaacckacaWGVbGaam OzaiaacckacaWGKbGaamyyaiaadMhacaWGZbaaa8aacqGHxdaTcaaI XaGaaGimaiaaicdaaaa@74E7@

FCR= Dry weight of feed given ( g ) Gain in wet weight of fish ( g ) MathType@MTEF@5@5@+= feaagKart1ev2aqatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq=Jc9 vqaqpepm0xbba9pwe9Q8fs0=yqaqpepae9pg0FirpepeKkFr0xfr=x fr=xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaqcfaieaaaaaa aaa8qacaWGgbGaam4qaiaadkfacqGH9aqpdaWcaaqaaiaadseacaWG YbGaamyEaiaacckacaWG3bGaamyzaiaadMgacaWGNbGaamiAaiaads hacaGGGcGaam4BaiaadAgacaGGGcGaamOzaiaadwgacaWGLbGaamiz aiaacckacaWGNbGaamyAaiaadAhacaWGLbGaamOBaiaacckadaqada WdaeaapeGaam4zaaGaayjkaiaawMcaaaqaaiaadEeacaWGHbGaamyA aiaad6gacaGGGcGaamyAaiaad6gacaGGGcGaam4DaiaadwgacaWG0b GaaiiOaiaadEhacaWGLbGaamyAaiaadEgacaWGObGaamiDaiaaccka caWGVbGaamOzaiaacckacaWGMbGaamyAaiaadohacaWGObGaaiiOam aabmaapaqaa8qacaWGNbaacaGLOaGaayzkaaaaaaaa@720C@

Survival ( % )= Final number of fish Initial number of fish ×100 MathType@MTEF@5@5@+= feaagKart1ev2aqatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq=Jc9 vqaqpepm0xbba9pwe9Q8fs0=yqaqpepae9pg0FirpepeKkFr0xfr=x fr=xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaqcfaieaaaaaa aaa8qacaWGtbGaamyDaiaadkhacaWG2bGaamyAaiaadAhacaWGHbGa amiBaiaacckadaqadaWdaeaapeGaaiyjaaGaayjkaiaawMcaaiabg2 da9maalaaabaGaamOraiaadMgacaWGUbGaamyyaiaadYgacaGGGcGa amOBaiaadwhacaWGTbGaamOyaiaadwgacaWGYbGaaiiOaiaad+gaca WGMbGaaiiOaiaadAgacaWGPbGaam4CaiaadIgaaeaacaWGjbGaamOB aiaadMgacaWG0bGaamyAaiaadggacaWGSbGaaiiOaiaad6gacaWG1b GaamyBaiaadkgacaWGLbGaamOCaiaacckacaWGVbGaamOzaiaaccka caWGMbGaamyAaiaadohacaWGObaaaiabgEna0kaaigdacaaIWaGaaG imaaaa@6F4A@

PER= Increment of body weight ( g ) Protein intake ( g ) MathType@MTEF@5@5@+= feaagKart1ev2aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq=Jc9 vqaqpepm0xbba9pwe9Q8fs0=yqaqpepae9pg0FirpepeKkFr0xfr=x fr=xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaqcfaOaamiuai aadweacaWGsbGaeyypa0ZaaSaaaeaaqaaaaaaaaaWdbiaadMeacaWG UbGaam4yaiaadkhacaWGLbGaamyBaiaadwgacaWGUbGaamiDaiaacc kacaWGVbGaamOzaiaacckacaWGIbGaam4BaiaadsgacaWG5bGaaiiO aiaadEhacaWGLbGaamyAaiaadEgacaWGObGaamiDaiaacckadaqada WdaeaapeGaam4zaaGaayjkaiaawMcaaaWdaeaapeGaamiuaiaadkha caWGVbGaamiDaiaadwgacaWGPbGaamOBaiaacckacaWGPbGaamOBai aadshacaWGHbGaam4AaiaadwgacaGGGcWaaeWaa8aabaWdbiaadEga aiaawIcacaGLPaaaaaaaaa@6606@

Statistical analysis was carried out using Analysis of Variance and Duncan Multiple Range tests to study the significant differences.

Results

Proximate composition of experimental diets

The proximate composition of the experimental diets fed to common carp fry is presented in Table 1. All the diets had about 30% protein and the values of crude protein ranged from 29.37% in T2 to 29.83% in T1.Moisture content of diets ranged from 4.07% in T0 to 4.47% in T2, but crude fat levels were ranged from 2.25% in T0 to 3.6% in T2. Similarly, the crude fibre content of diets ranged from 4.82% in T0 to 5.96% in T2. Overall, the proximate compositions of the experimental diets indicate that there was no significant difference between the diets after incorporation of betaine hydrochloride.

Components

Diets

T0

T1

T2

Moisture (%)

4.07±0.18

4.4±0.89

4.47±0.02

Dry matter (%)

95.93±0.36

95.6±0.06

95.53±0.06

Crude protein (%)

29.67±0.27

29.83±0.056

29.37±0.76

Crude fat (%)

2.25±2.52

3.1±2.53

3.6±1.5

Crude fibre (%)

4.82±1.25

5.37±0.45

5.96±0.5

Ash (%)

19.15±1.65

17.75±2.54

17.65±1.20

NFE (%)

39.88

39.31

38.31

Table 1: Proximate composition of experimental diets (Mean±SD).

Water quality parameters

During the experimental period, the water temperature ranged from 25 to 28.8 °C, pH from 6.5 to 7.8, dissolved oxygen from 6.4 to 8.6 mg/l, free carbon dioxide from 0.15 to 4.8 mg/l, total alkalinity from 31 to 67.2 mg/l of CaCO3, and ammonia-nitrogen from 0.02 to 0.16μg at N/l. These water quality parameters recorded during the experimental period were found suitable and well within the rangesuitable for common carp culture. Further, it was also observed that the incorporation of betaine in diet did not affect the tank water.

Growth studies

Data on growth, survival, food conversion ratio and protein efficiency ratio of common carp fed on the graded levels of betaine incorporated diets are given in Table 2. Highest growth in terms of specific growth rate was observed in 0.25% (T1) betaine incorporated diet fed common carp, compared to T0 and T2. Similarly, highest survival (83.33%) and PER (0.012) were recorded in T1 treatment, which also recorded low FCR (1.69). However, it was observed that there were no significant difference in experimental groups in terms of specific growth rate and PER, but there were significant differences in survival and FCR of feed treatments.

Parameters

Diets

T0

T1

T2

Survival (%)

69.99±4.71

83.33±4.70

73.33±9.43

SGR (% / day)

0.39

0.51

0.42

FCR

2.2

1.69

2.05

PER

0.009

0.012

0.009

Table 2: Survival, SGR, FCR and PER of Cyprinus carpio fed graded levels of betaine incorporated diets (Mean  SD).

Discussion

Growth, survival, food conversion ratio, protein efficiency ratio were best in the treatment containing 0.25 % betaine [4]. Addition of a commercial feed attractant, Aquasavor, produced better growth in Catlacatla when provided at a level of 2% [14]. Dimethyl- beta-propiothetine (DMPT) and betaine increased body weight and molting rate in Penaeusindicus [3]. In striped bass, feed conversion improved in animals fed a fishmeal- based diet supplemented with a feeding stimulant mixture of several amino acids and betaine at rate of 2.7 % of the diet [7,15] Reviewed research pertaining to dietary nucleotide application to sea bream larvae and hypothesized that an exogenous supply of nucleotides may promote growth of fish on early stages. The weight gain of fish fed the betaine supplemented diet was significantly higher than that of fish fed the basal diet [16]. Dietary choline betaine supplementation resulted in a significant increase in a feed consumption and weight gain in tilapia [17] while betaine and amino acids had additive effects in striped bass [5].

A betaine supplemented diet resulted in enhanced feed preference in juvenile Gibel carp [18] when supplemented with feeding stimulant finnistim, a betaine containing palatability enhancer. Where in, 66 % of the fish meal in rainbow trout feeds was replaced by rapeseed protein concentrates without significant reduction in feed intake and growth although when 100 % of the fish meal was replaced, fish growth was reduced [19]. Stimulant supplementation can affect not only feed intake and subsequently weight gain but also food conversion ratio indicating an overall increase in diet efficiency [6]. Growth, food conversion ratio, protein retention efficiency and energy retention efficiency were improved in rainbow trout fed plant protein diets containing the feeding stimulant taurine [20]. Growth, specific growth rate and protein efficiency ratio were significantly better in fish fed 50 mg/kg than in the control [21]. Turan & Akyurt [22] also obtained significantly higher growth in African catfish (Clariasgariepinus) fed and rostenedione based diets. It may be concluded that dietary supplementation of betaine enhanced growth and FCR in common carp, which help to reduce feed wastage while enhancing the feed utilization.

Acknowledgements

The authors are thankful to Head of the Department of Aquaculture and field staff of the College of Fisheries, Mangalore, for their help in carrying out the study.

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