Journal of ISSN: 2469 - 2786 JBMOA

Bacteriology & Mycology: Open Access
Research Article
Volume 3 Issue 3 - 2016
Antibiotic Susceptibility Patterns of Aeromonas Species Isolated from Water and Stool Samples in Mthatha Region Eastern Cape Province of South Africa
Muringani BN*, Obi CL, Apalata T and Vasaikar SD
Department of Medical Microbiology, Walter Sisulu University, South Africa
Received: May 06, 2015 | Published: December 19, 2016
*Corresponding author: Muringani BN, Department of Medical Microbiology, Faculty of Health Sciences, Walter Sisulu University, Mthatha, South Africa, Tel: +263775939074; Email:
Citation: Muringani BN, Obi CL, Apalata T, Vasaikar SD (2016) Antibiotic Susceptibility Patterns of Aeromonas Species Isolated from Water and Stool Samples in Mthatha Region Eastern Cape Province of South Africa. J Bacteriol Mycol Open Access 3(3): 00066. DOI: 10.15406/jbmoa.2016.03.00066

Abstract

Objective: To determine the antibiotic susceptibility patterns of species isolated from water and stool samples in Mthata Region.

Materials and methods: A study was undertaken to determine the presence and susceptibility profiles of Aeromonas species isolated from water and stool samples submitted to Mthata Government hospital from January 2012- October 2012.A total of 500 water samples and 150 stool samples were analysed.

Results: Aeromonas species were found in 30% of the water samples and in 10% of the stool samples. 20% of the isolates from the water samples were β-lactamase positive and 100% of the stool isolates respectively, giving a total of 45 isolates which were ESBL positive. The isolates were susceptible to ciprofloxacin, imipenem and tazobactum and the ESBL strains presented with variable resistance to all the antibiotics and were mostly resistant to β-lactams. Table 1 shows the comparisons of the susceptibility profiles of Aeromonas species from water and stool samples. The results show a similar trend in susceptibility profile of Aeromonas isolated from water and stool samples implicating a likely hood of cross contamination due to contaminated water or the population contaminating the water sources.

Conclusion: There is need for laboratories to expand their screening methods and include routine checking of Aeromonas species in patients with diarrhea. While this research needs to further evaluate the Phylogenetic characteristics of the isolated strains in order to link the sources of contamination.

Keywords: Aeromonas species; Kirby bauer disc diffusion Method; Diarrhea; ESBL; Septiceamia; Endocarditis; Peritonitis; Osteomylitis

Introduction

Aeromonas species are motile gram negative rods of the family Aeromonadaceae. They are emerging human pathogens suspected to cause gastroenteritis ranging from mild enteritis to cholera -like diarrhea, known as travelers diarrhea. Aeromonas species have been reported to cause infections such as septiceamia, endocarditis, peritonitis, osteomylitis, myonecrosis, haemolytic uraemic syndrome, meningitis, respiratory tract diseases and ocular infection [1]. In addition to humans, they are also important pathogens in amphibians, repitiles and fish causing major problems in fish farming. Aeromonas species have also been isolated from a variety of foods such as vegetables, raw milk, icecream, meat and seafood [2,3]. They are known to form biofilms in chlorinated water pipes and this also serves as a source of human contamination. Its pathogenicity is due to the production of exotoxins (heamolysins and enterotoxins). Aeromonas spp are becoming increasingly resistant to multiple antibiotics. Such resistance could lead to serious clinical sequelae. Rate of faecal carriage in asymptomatic persons ranges from 0%-4%, while the isolation rate from individuals with diarrhea ranges from 0.8-7.4% [1,4]. It is commonly isolated from drinking water and seasonal relationship between presence in drinking water and in stools of patients with gastroenteritis have been reported [5,6].

Materials and Methods

500 Water samples and 150 stool samples were analysed between January -October 2012. Unformed stools submitted to NHLS M that was used.

Microbiological tests

Water samples were first inoculated onto selenite F broth and then inoculated onto blood agar supplemented with Ampicillin and a selective media cefsulodin-irgason-novobiocin agar (CIN). Stool samples were inoculated directly onto CIN and ampicillin blood agar. The cultures were incubated for 24-48hours aerobically at 37ËšC. An oxidase test was done on all bacilli colonies and hippurate hydrolysis test was done to differentiate the Aeromonas species. β-lactamase test was done on all isolates using a calorimetric test strip.

Susceptibility testing

Kirby bauer disc diffusion Method was used for susceptibility testing. The following antibiotics were used, Ampicillin, Gentamicin, Ciprofloxacin, Imipenem, Cotrimoxazole, piperacillin/tazobactum, chloramphinicol, ciphalothin and erythromycin. NCCLS 2012 Standards were used.

Results

Aeromonas species were isolated in 30% of the water samples and in 10% of the stool samples. A hydrophilia of the isolates from water samples, 20% were ESBL strains and 100% of the stool isolates respectively. All strains were resistant to Ampicillin but susceptible to Ciprofloxacin, imipenem, piperacillin/tazobactum. Their susceptibility varied on erythromycin, chloramphinicol, gentamicin and ciphalothin as shown in Table 1.

Antimicrobial Agents

Water Isolates

Stool Isolates

N=150

N=30

Ampicillin

0

0

Gentamicin

0

0

Ciprofloxacin

150

30

Imipenem

150

30

Cotrimoxazole

150

10

Piperacillin/Tazobactum

90

20

Chloramphinicol

80

15

Ciphalothin

150

20

Erythromycin

60

10

Table 1: Comparison of Susceptibility profiles of Aeromonas species from water and stool.

Conclusion

The results were as expected as all the strains were resistant to Ampicillin due to the production of β-lactamases and were sensitive to third -generation Cephalothins and were in accordance to results from findings from other researchers [1]. He justifies the need to include laboratory isolation techniques for Aeromonas species in unformed stool samples and also to screen for species in water sources. More work needs to be done in order to compare the Phylogenetic characteristics of isolates from water and stool samples so that we can be able to link the relationship between environment and human diseases due to Aeromonas in patients presenting with diarrhea/gastroenteritis.

References

  1. Igbinosa IH, Igumbor EU, Aghdasi F, Tom M, Okoh AI (2012) Emerging Aeromonas species infections and their significance in public health. Scientific World Journal pp. 625023.
  2. Rose BE, Okrend AJG (1998) USDA/FSIS Microbiology Laboratory Guidebook. (3rd edn), Chapter 7, Isolation and Identification, pp. 1-7.
  3. Taylor DN, Gardner P, Nichols RL, Ramsey KM, Smith LG, et al. (2003) Infectious Diseases. National foundation of infectious diseases 6(1): 1-6.
  4. Chopra AK, Graf J, Horneman AJ, Johnson JA (2009) Virulence factor - activity relationships (VFAR) with specific emphasis on Aeromonas species (spp.). J Water Health 7(Suppl 1): 29-54.
  5. Leclerc H (2003) Relationships between common water bacteria and pathogens in drinking water. IWA Publishing, London, UK.
  6. Sears CL, Kaper JB (1996) Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion. Microbiol Rev 60(1): 167-215.
© 2014-2016 MedCrave Group, All rights reserved. No part of this content may be reproduced or transmitted in any form or by any means as per the standard guidelines of fair use.
Creative Commons License Open Access by MedCrave Group is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at http://medcraveonline.com
Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version | Opera |Privacy Policy