ISSN: 2378-315X BBIJ

Biometrics & Biostatistics International Journal
Proceeding
Volume 5 Issue 1 - 2017
Bedsore Revitalization by- LLLT, Low Level Laser (LED- Ga-Al- As 660) Therapy
Mohammad Nazrul Islam*, Golam Abu Zakaria, kazi Shamimuzzaman and Quamrul Akhter Sanju
Shaheed Suhrawardy Medical College Hospital, Bangladesh
Received: October 16, 2016| Published: January 27, 2017
*Corresponding author: Mohammad Nazrul Islam, Head - Bio-medical and Medical Biotechnology Dept. Shaheed Suhrawardy Medical College Hospital. Dhaka-1207, Bangladesh, Tel: CELL: +88-01733381819; Email: ;
Citation: Islam MN, Zakaria GA, Shamimuzzaman K, Sanju QA (2017) Bedsore Revitalization by- LLLT, Low Level Laser (LED- Ga-Al- As 660) Therapy. Biom Biostat Int J 5(1): 00123. DOI: 10.15406/bbij.2017.05.00123
Keywords: Bedsore healing; Soft tissue healing; Decubitus ulcer healing; Low level laser; Wound healing

Background

In 1967 a few years after the first working laser was invented, Endre Mester in Semmelweis University Budapest, Hungary wanted to find out if laser might cause cancer. He took some mice, shaved the hair off their backs, divided them into two groups and gave a laser treatment with a low powered ruby laser to one group. They did not get cancer and to his surprise the hair on the treated group grew back more quickly than the untreated group. That was how "laser biostimulation" was discovered.

Purpose of the work

The effects of pulsed monochromatic light, with fixed pulsations and wavelengths, on the healing of pressure ulcers were evaluated in this prospective, randomized, controlled study.

Approach and Methodology

A placebo-controlled, double-blind study using low energy photon therapy (LLLT) was performed in ten patients with bedsore on the back. Treatment was given three times a week for 10 weeks, using monochromatic (red) optical sources; diode 660nm (GaAl-660). The patients who were randomized to placebo treatment received sham therapy from an identical-appearing light source from the same delivery system.

Results

Ten patients with 10 bedsore were randomized to receive LLLT or placebo therapy. At the conclusion of the study, the percentage of the initial ulcer area remaining unhealed in the LLLT and placebo groups was 24.4% and 84.7%, respectively (P = 0.0008). The decrease in ulcer area (compared to baseline) observed in the LLLT and placebo groups was 193.0 mm2 and 14.7 mm2, respectively (P = 0.0002).

One patient dropped out of the study, complaining of lack of treatment efficacy; he was found to be randomized to the placebo group. There were no adverse effects.

Figure 1: Chronological Picture View of a Laser (LLLT) Treated Patient.

Conclusion

In this placebo-controlled, double-blind study LLLT was an effective modality for the treatment of bedsore which were resistant to conventional medical management.

The results are encouraging as pulsed monochromatic light increased healing rate and shortened healing time. This will positively affect the quality of life in elderly patients with pressure ulcers [1-57].

References

  1. Michael R Hamblin, Tatiana N Demidova (2006) Mechanisms of Low Level Laser Therapy. Harvard Medical School 6140.
  2. Pereira AN, Eduardo Cde P, Matson E, Marques MM. (2002) Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. Lasers Surg Med 31(4): 263-267.
  3. Sutherland JC (2002) Biological effects of polychromatic light. Photochem Photobiol 76(2):164-170.
  4. Karu T (1989) Laser biostimulation: a photobiological phenomenon. J Photochem Photobiol B 3(4): 638-640.
  5. Karu TI, Afanaseva NI (1995) Cytochrome c oxidase as the primary photoacceptor upon laser exposure of cultured cells to visible and near IR-range light. Dokl Akad Nauk 342(5): 693-695.
  6. Capaldi R A, Malatesta F, Darley Usmar V M (1983) Structure of cytochrome c oxidase. Biochim Biophys Acta 726: 135-148.
  7. Szundi I, Liao GL, Einarsdottir O (2001) Near-infrared time-resolved optical absorption studies of the reaction of fully reduced cytochrome c oxidase with dioxygen. Biochemistry 40(8): 2332-2339.
  8. Karu TI, Kolyakov SF (2005) Exact action spectra for cellular responses relevant to phototherapy. Photomed Laser Surg 23(4): 355-361.
  9. Yu W, Naim JO, McGowan M, Ippolito K, Lanzafame RJ (1997) Photomodulation of oxidative metabolism and electron chain enzymes in rat liver mitochondria. Photochem Photobiol 66(6): 866-871.
  10. Passarella S (1989) He-Ne laser irradiation of isolated mitochondria. J Photochem Photobiol B 3(4): 642-643.
  11. Friedmann H, Lubart R, Laulicht I, Rochkind S (1991) A possible explanation of laser-induced stimulation and damage of cell cultures. J Photochem Photobiol B 11(1): 87-91.
  12. Eichler M, Lavi R, Shainberg A, Lubart R (2005) Flavins are source of visible-light-induced free radical formation in cells. Lasers Surg Med 37(4):314-319.
  13. Plaetzer K, Kiesslich T, Krammer B, Hammerl P (2002) Characterization of the cell death modes and the associated changes in cellular energy supply in response to AlPcS4-PDT. Photochem Photobiol Sci 1(3): 172-177.
  14. Lubart R, Eichler M, Lavi R, Friedman H, Shainberg A (2005) Low-energy laser irradiation promotes cellular redox activity. Photomed Laser Surg 23(1): 3-9.
  15. Duan R, Liu TC, Li Y, Guo H, Yao LB (2001) Signal transduction pathways involved in low intensity He-Ne laser-induced respiratory burst in bovine neutrophils: a potential mechanism of low intensity laser biostimulation. Lasers Surg Med 29(2): 174-178.
  16. Antunes F, Boveris A, Cadenas E (2004) On the mechanism and biology of cytochrome oxidase inhibition by nitric oxide.   Proc Natl Acad Sci U S A 101(48): 16774-16779.
  17. Karu TI, Pyatibrat LV, Afanasyeva NI (2005) Cellular effects of low power laser therapy can be mediated by nitric oxide. Lasers Surg Med 36(4): 307-314.
  18. Moriyama Y, Moriyama EH, Blackmore K, Akens MK, Lilge L (2005) In Vivo Study of the Inflammatory Modulating Effects of Low-level Laser Therapy on iNOS Expression Using Bioluminescence Imaging. Photochem Photobiol 81(6): 1351-1355.
  19. Schafer FQ, Buettner GR (2001) Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radic Biol Med 30(11): 1191-1212.
  20. Liu H, Colavitti R, Rovira II, Finkel T (2005) Redox-dependent transcriptional regulation. Circ Res 97(10): 967-974.
  21. Yang M, Nazhat N B, Jiang X, Kelsey S M, Blake DR, et al. (1996) Adriamycin stimulates proliferation of human lymphoblastic leukaemic cells via a mechanism of hydrogen peroxide (H2O2) production. Br J Haematol 95(2): 339-344.
  22. Kirlin WG, Cai J, Thompson SA, Diaz D, Kavanagh TJ, et al. (1999) Glutathione redox potential in response to differentiation and enzyme inducers. Free Radic Biol Med 27(11-12): 1208-1218.
  23. Alaluf S, Muir Howie H, Hu HL, Evans A, Green MR (2000) Atmospheric oxygen accelerates the induction of a post-mitotic phenotype in human dermal fibroblasts: the key protective role of glutathione. Differentiation 66(2-3):147-155.
  24. Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 49(1): 1-17.
  25. Young S, Bolton P, Dyson M, Harvey W, Diamantopoulos C (1989) Macrophage responsiveness to light therapy. Lasers Surg Med 9(5): 497-505.
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